![IHC-P analysis of colon adenocarcinoma tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of colon adenocarcinoma tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_1868_IHC-P_w_23061420_801.webp)
IHC-P analysis of colon adenocarcinoma tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
PKA R2 antibody [4A3]
GTX83825
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRKAR2A
Overview
- SupplierGeneTex
- Product NamePKA R2 antibody [4A3] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4A3
- Concentration0.6 mg/ml
- ConjugateUnconjugated
- Gene ID5576
- Target namePRKAR2A
- Target descriptionprotein kinase cAMP-dependent type II regulatory subunit alpha
- Target synonymscAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase type II-alpha regulatory subunit; PKR2; PRKAR2; protein kinase A, RII-alpha subunit; protein kinase, cAMP-dependent, regulatory subunit type II alpha
- HostMouse
- IsotypeIgG1
- Protein IDP13861
- Protein NamecAMP-dependent protein kinase type II-alpha regulatory subunit
- Scientific DescriptionType II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of human ovary adenocarcinoma tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_1869_IHC-P_w_23061420_705.webp)
![IHC-P analysis of breast tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of breast tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_1870_IHC-P_w_23061420_515.webp)
![IHC-P analysis of liver carcinoma tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of liver carcinoma tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_1871_IHC-P_w_23061420_270.webp)
![IHC-P analysis of kidney tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of kidney tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_1872_IHC-P_w_23061420_345.webp)
![IHC-P analysis of liver tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of liver tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_1873_IHC-P_w_23061420_545.webp)
![IHC-P analysis of pancreas tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of pancreas tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_1874_IHC-P_w_23061420_443.webp)
![IHC-P analysis of prostate tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of prostate tissue using GTX83825 PKA R2 antibody [4A3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_1875_IHC-P_w_23061420_465.webp)
![WB analysis of wild type and PKA R2 knock out HeLa cell lysate(10microg per lane) using GTX83825 PKA R2 antibody [4A3]. Dilution : 1:2000 WB analysis of wild type and PKA R2 knock out HeLa cell lysate(10microg per lane) using GTX83825 PKA R2 antibody [4A3]. Dilution : 1:2000](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_3929_20181228_WB_w_23061420_956.webp)
![WB analysis of HEK293T cells transfected with PKA R2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83825 PKA R2 antibody [4A3]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with PKA R2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83825 PKA R2 antibody [4A3]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83825/GTX83825_3930_WB_w_23061420_589.webp)


![IHC-P analysis of colon adenocarcinoma tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_1862_IHC-P_w_23061420_818.webp)
![Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser99) antibody [GT1304] (GTX03216) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03216/GTX03216_4000001579_20210716_WB_CIP_w_23053123_534.webp)

