More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PKC alpha beta II (Phospho-Thr638 641) Antibody Cy3 Conjugated

PKC alpha beta II (Phospho-Thr638 641) Antibody Cy3 Conjugated

MBS9461272
MyBioSource
Product group Antibodies
Price on request
0.1 ml
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    MyBioSource
  • Product Name
    PKC alpha beta II (Phospho-Thr638 641) Antibody Cy3 Conjugated
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Other Conjugate
  • Estimated Purity
    Purified by Protein A
  • Isotype
    IgG
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Anti-PKC-pan Antibody
Product group Antibodies
Antibodies.com
Anti-PKC-pan AntibodyA94967
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Abbexa
PKC alpha (Phospho-Tyr657) AntibodyABX012471
ApplicationsWestern Blot, ELISA
  • SizePrice
Figure 1. Western blot analysis of PKC Alpha/PRKCA using anti-PKC Alpha/PRKCA antibody (A00743-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified polyclonal antibody (Catalog # A00743-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC Alpha/PRKCA at approximately 80 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.
Product group Antibodies
Boster Bio
Anti-PKC Alpha/PRKCA Antibody Picoband(r)A00743-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PRKCA Antibody144-00267
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPRKCA
  • SizePrice
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Product group Antibodies
References
Bioss
Phospho-PKC alpha (Thr638) Polyclonal AntibodyBS-3333R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Rat
TargetPRKCA
  • SizePrice
Western Blot analysis of MCF-7 cells using PKC Polyclonal Antibody
Product group Antibodies
Cusabio
PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ AntibodyCSB-PA003800
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Product group Antibodies
Biomatik
Prkca Polyclonal AntibodyCAC09189
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetPRKCA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PRKCA / PKC-Alpha AntibodyLS-C402927
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-PRKCA antibody. Corresponding PRKCA RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-PRKCA AntibodyHPA006564
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PKC alpha(GTX111096) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
GeneTex
PKC alpha antibody [C1C3]GTX111096
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetPRKCA
  • SizePrice