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ProductsBack/PKC eta Antibody (YA126, Rabbit)

PKC eta Antibody (YA126, Rabbit)

HY-P80869
MedChem Express
TargetPRKCH
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PKC eta Antibody (YA126, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5583
  • Target name
    PRKCH
  • Target description
    protein kinase C eta
  • Target synonyms
    PKC-L, PKCL, PRKCL, nPKC-eta, uORF2, protein kinase C eta type, Protein uPEP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PKC eta Antibody (YA126) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKC eta.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25798 PKC eta (phospho Thr655) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptide corresponding to the immunogen from other PKC isoforms (Lane 5-9). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 10) eliminates the signal further verifying that the antibody is phospho-specific. Dilution : 1:2000
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Figure 1. Western blot analysis of PKC eta using anti-PKC eta antibody (PB9556). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate Lane 4: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC eta antigen affinity purified polyclonal antibody (Catalog # PB9556) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC eta at approximately 78KD. The expected band size for PKC eta is at 78KD.
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