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ProductsBack/PKC eta (phospho Thr655) antibody
WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25798 PKC eta (phospho Thr655) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptide corresponding to the immunogen from other PKC isoforms (Lane 5-9). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 10) eliminates the signal further verifying that the antibody is phospho-specific. Dilution : 1:2000
WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25798 PKC eta (phospho Thr655) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptide corresponding to the immunogen from other PKC isoforms (Lane 5-9). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 10) eliminates the signal further verifying that the antibody is phospho-specific. Dilution : 1:2000
WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25798 PKC eta (phospho Thr655) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptide corresponding to the immunogen from other PKC isoforms (Lane 5-9). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 10) eliminates the signal further verifying that the antibody is phospho-specific. Dilution : 1:2000

PKC eta (phospho Thr655) antibody

GTX25798
GeneTex
ApplicationsFlow Cytometry, Western Blot
DownloadDatasheet
Product group Antibodies
ReactivityHuman
TargetPRKCH
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Overview

  • Supplier
    GeneTex
  • Product Name
    PKC eta (phospho Thr655) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Antibody Specificity
    This antibody does not cross-react with any other PKC isoforms tested.
  • Application Supplier Note
    WB: 1:2000. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5583
  • Target name
    PRKCH
  • Target description
    protein kinase C eta
  • Target synonyms
    nPKC-eta; PKCL; PKC-L; PRKCL; protein kinase C eta type
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24723
  • Protein Name
    Protein kinase C eta type
  • Scientific Description
    Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Development of triazole-based PKC-inhibitors to overcome resistance to EGFR inhibitors in EGFR-mutant lung cancers.
    Read more