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PRKCH Antibody

PACO33648
Assay Genie
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPRKCH
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PRKCH Antibody (PACO33648)
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5583
  • Target name
    PRKCH
  • Target description
    protein kinase C eta
  • Target synonyms
    PKC-L, PKCL, PRKCL, nPKC-eta, uORF2, protein kinase C eta type, Protein uPEP2
  • Host
    Rabbit
  • Protein IDP24723
  • Protein Name
    Protein kinase C eta type
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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MSDS

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Western Blot Positive WB detected in: HEK293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Rat heart tissue, Rat kideny tissue, Rat stomach tissue, Mouse heart tissue, Mouse kidney tissue, Mouse stomach tissue All lanes: PRKCH antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 78, 60 kDa Observed band size: 78 kDa
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WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25798 PKC eta (phospho Thr655) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptide corresponding to the immunogen from other PKC isoforms (Lane 5-9). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 10) eliminates the signal further verifying that the antibody is phospho-specific. Dilution : 1:2000
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Figure 1. Western blot analysis of PKC eta using anti-PKC eta antibody (PB9556). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate Lane 4: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC eta antigen affinity purified polyclonal antibody (Catalog # PB9556) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC eta at approximately 78KD. The expected band size for PKC eta is at 78KD.
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