![PKC proteins were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Ser695) antibody [HL3397] (GTX641234) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. PKC proteins were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Ser695) antibody [HL3397] (GTX641234) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641234/GTX641234_T-45579_20241227_WB_Ag_24123021_807.webp)
PKC proteins were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Ser695) antibody [HL3397] (GTX641234) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PKC theta (phospho Ser695) antibody [HL3397]
GTX641234
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPRKCQ
Overview
- SupplierGeneTex
- Product NamePKC theta (phospho Ser695) antibody [HL3397]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3397
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5588
- Target namePRKCQ
- Target descriptionprotein kinase C theta
- Target synonymsPRKCT, nPKC-theta, protein kinase C theta type
- HostRabbit
- IsotypeIgG
- Protein IDQ04759
- Protein NameProtein kinase C theta type
- Scientific DescriptionProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Ser695) antibody [HL3397] (GTX641234) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Ser695) antibody [HL3397] (GTX641234) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641234/GTX641234_45698_20250408_WB_treatment_PMA_25041720_814.webp)






![PKC proteins (10 ng) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Ser695) antibody [HL3396] (GTX641233) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641233/GTX641233_T-45579_20241122_WB_Ag_24112622_757.webp)