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Placental Alkaline Phosphatase(PLAP) Monoclonal Antibody

RD6210
Reddot Biotech
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetALPP
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    Placental Alkaline Phosphatase(PLAP) Monoclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Concentration
    100 ug/ml
  • Gene ID250
  • Target name
    ALPP
  • Target description
    alkaline phosphatase, placental
  • Target synonyms
    ALP, ALPI, IAP, PALP, PLAP, PLAP-1, alkaline phosphatase, placental type, Intestinal alkaline phosphatase, Intestinal-type alkaline phosphatase, alkaline phosphatase Regan isozyme, alkaline phosphomonoesterase, glycerophosphatase, placental alkaline phosphatase 1
  • Host
    Mouse
  • Scientific Description
    Placental Alkaline Phosphatase(PLAP) can be expressed as carcinoembryonic antigen in some tumors of reproductive system, gastrointestinal tract and lung cancer. This antibody is mainly used to label the cells of placental trophoblastic tumor, ovarian germ cell tumor and testicular seminoma.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALPP using anti-ALPP antibody (A01718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALPP antigen affinity purified polyclonal antibody (Catalog # A01718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALPP at approximately 70 kDa. The expected band size for ALPP is at 58 kDa.
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