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ProductsBack/Pleckstrin (PLEK) Mouse Monoclonal Antibody [Clone ID: OTI4G6]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEK (RC203780, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEK. Positive lysates LY419171 (100ug) and LC419171 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEK (RC203780, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEK. Positive lysates LY419171 (100ug) and LC419171 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEK (RC203780, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEK. Positive lysates LY419171 (100ug) and LC419171 (20ug) can be purchased separately from OriGene.

Pleckstrin (PLEK) Mouse Monoclonal Antibody [Clone ID: OTI4G6]

CF500891
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPLEK
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Pleckstrin (PLEK) Mouse Monoclonal Antibody [Clone ID: OTI4G6]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4G6
  • Gene ID5341
  • Target name
    PLEK
  • Target description
    pleckstrin
  • Target synonyms
    P47, PLEK1, pleckstrin, platelet 47 kDa protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP08567
  • Protein Name
    Pleckstrin
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PLEK mouse monoclonal antibody, clone OTI4G6 (formerly 4G6)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Pleckstrin/PLEK using anti-Pleckstrin/PLEK antibody (A06656-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleckstrin/PLEK antigen affinity purified polyclonal antibody (Catalog # A06656-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleckstrin/PLEK at approximately 40 kDa. The expected band size for Pleckstrin/PLEK is at 40 kDa.
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