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ProductsBack/PLG / Plasmin / Plasminogen Antibody (aa611-660)

PLG / Plasmin / Plasminogen Antibody (aa611-660)

LS-B11999
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPLG
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PLG / Plasmin / Plasminogen Antibody (aa611-660)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Western Blot: Suggested dilution at 1.0microg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.. IHC, IHC-P (10 µg/ml), WB (0.2 - 1 µg/ml) Western Blot: Suggested dilution at 1.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5340
  • Target name
    PLG
  • Target description
    plasminogen
  • Target synonyms
    HAE4, plasminogen, plasmin
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PLG using anti-PLG antibody (A00702-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse spleen tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLG antigen affinity purified polyclonal antibody (Catalog # A00702-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLG at approximately 90-100 kDa. The expected band size for PLG is at 90-100 kDa.
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