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Lane 1: U-87MG cell lysates; Lane 2: K562 cell lysates; Lane 3: Hela cell lysates; Lane 4: A431 cell lysates probed with PLOD1 Monoclonal Antibody, Unconjugated (bsm-51740M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: U-87MG cell lysates; Lane 2: K562 cell lysates; Lane 3: Hela cell lysates; Lane 4: A431 cell lysates probed with PLOD1 Monoclonal Antibody, Unconjugated (bsm-51740M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: U-87MG cell lysates; Lane 2: K562 cell lysates; Lane 3: Hela cell lysates; Lane 4: A431 cell lysates probed with PLOD1 Monoclonal Antibody, Unconjugated (bsm-51740M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

PLOD1 Monoclonal Antibody

BSM-51740M
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetPLOD1
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Overview

  • Supplier
    Bioss
  • Product Name
    PLOD1 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(WB=1:500-2000，)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M4G1
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID5351
  • Target name
    PLOD1
  • Target description
    procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
  • Target synonyms
    EDS6, EDSKCL1, LH, LH1, LLH, PLOD, procollagen-lysine,2-oxoglutarate 5-dioxygenase 1, lysine hydroxylase, lysyl hydroxlase 1, procollagen lysyl hydroxylase 1, procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
  • Host
    Mouse
  • Protein IDQ02809
  • Protein Name
    Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLOD1 using anti-PLOD1 antibody (A05322-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLOD1 antigen affinity purified polyclonal antibody (Catalog # A05322-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLOD1 at approximately 84 kDa. The expected band size for PLOD1 is at 84 kDa.
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