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Anti-PLOD1 Antibody

CAB7919
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPLOD1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-PLOD1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID5351
  • Target name
    PLOD1
  • Target description
    procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
  • Target synonyms
    EDS6, EDSKCL1, LH, LH1, LLH, PLOD, procollagen-lysine,2-oxoglutarate 5-dioxygenase 1, lysine hydroxylase, lysyl hydroxlase 1, procollagen lysyl hydroxylase 1, procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
  • Host
    Rabbit
  • Protein IDQ02809
  • Protein Name
    Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
  • Scientific Description
    PLOD1 Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLOD1 using anti-PLOD1 antibody (A05322-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLOD1 antigen affinity purified polyclonal antibody (Catalog # A05322-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLOD1 at approximately 84 kDa. The expected band size for PLOD1 is at 84 kDa.
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Lane 1: U-87MG cell lysates; Lane 2: K562 cell lysates; Lane 3: Hela cell lysates; Lane 4: A431 cell lysates probed with PLOD1 Monoclonal Antibody, Unconjugated (bsm-51740M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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WB analysis of various sample lysates using GTX32707 LH1 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25μg per lane
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