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ProductsBack/PMS2 Mouse Monoclonal Antibody [Clone ID: 349.29.5.2]
WB analysis using Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. The blot was incubated with a 1:1,000 dilution of the antibody at RTfollowed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemicals SuperSignal™ substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein.
WB analysis using Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. The blot was incubated with a 1:1,000 dilution of the antibody at RTfollowed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemicals SuperSignal™ substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein.
WB analysis using Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. The blot was incubated with a 1:1,000 dilution of the antibody at RTfollowed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemicals SuperSignal™ substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein.

PMS2 Mouse Monoclonal Antibody [Clone ID: 349.29.5.2]

TA319565
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human, Mouse, Primate, Rat
TargetPMS2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PMS2 Mouse Monoclonal Antibody [Clone ID: 349.29.5.2]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    349.29.5.2
  • Gene ID5395
  • Target name
    PMS2
  • Target description
    PMS1 homolog 2, mismatch repair system component
  • Target synonyms
    HNPCC4, LYNCH4, MLH4, MMRCS4, PMS-2, PMS2CL, PMSL2, mismatch repair endonuclease PMS2, DNA mismatch repair protein PMS2, PMS1 homolog 2, mismatch repair protein, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2
  • Host
    Mouse
  • Protein IDP54278
  • Protein Name
    Mismatch repair endonuclease PMS2
  • Scientific Description
    Mouse monoclonal anti-PMS2 antibody
  • Reactivity
    Hamster, Human, Mouse, Primate, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PMS2 using anti-PMS2 antibody (A01028). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMS2 antigen affinity purified polyclonal antibody (Catalog # A01028) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMS2 at approximately 115KD. The expected band size for PMS2 is at 96KD.
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