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Western Blot analysis of HT29 cells using Pinin Polyclonal Antibody
Western Blot analysis of HT29 cells using Pinin Polyclonal Antibody
Western Blot analysis of HT29 cells using Pinin Polyclonal Antibody

PNN Antibody

CSB-PA003786
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPNN
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Overview

  • Supplier
    Cusabio
  • Product Name
    PNN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5411
  • Target name
    PNN
  • Target description
    pinin, desmosome associated protein
  • Target synonyms
    140 kDa nuclear and cell adhesion-related phosphoprotein; desmosome-associated protein; domain-rich serine protein; DRS; DRSP; melanoma metastasis clone A protein; memA; neutrophil protein; nuclear protein SDK3; pinin; SDK3; SR-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H307
  • Protein Name
    Pinin
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PNN/DRSP using anti-PNN/DRSP antibody (A01590-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNN/DRSP antigen affinity purified polyclonal antibody (Catalog # A01590-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNN/DRSP at approximately 82KD. The expected band size for PNN/DRSPis at 82KD.
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