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WB analysis of human B lymphoblast cells using GTX45055 PNN antibody at 0.2-1microg/ml.
WB analysis of human B lymphoblast cells using GTX45055 PNN antibody at 0.2-1microg/ml.
WB analysis of human B lymphoblast cells using GTX45055 PNN antibody at 0.2-1microg/ml.

PNN antibody, Internal

GTX45055
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPNN
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Overview

  • Supplier
    GeneTex
  • Product Name
    PNN antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5411
  • Target name
    PNN
  • Target description
    pinin, desmosome associated protein
  • Target synonyms
    DRS, DRSP, SDK3, memA, pinin, 140 kDa nuclear and cell adhesion-related phosphoprotein, SR-like protein, desmosome-associated protein, domain-rich serine protein, melanoma metastasis clone A protein, neutrophil protein, nuclear protein SDK3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H307
  • Protein Name
    Pinin
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PNN/DRSP using anti-PNN/DRSP antibody (A01590-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNN/DRSP antigen affinity purified polyclonal antibody (Catalog # A01590-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNN/DRSP at approximately 82KD. The expected band size for PNN/DRSPis at 82KD.
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