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ProductsBack/PNN Antibody - middle region (OAAB07802)

PNN Antibody - middle region (OAAB07802)

OAAB07802
Aviva Systems Biology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPNN
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    PNN Antibody - middle region (OAAB07802)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID5411
  • Target name
    PNN
  • Target description
    pinin, desmosome associated protein
  • Target synonyms
    140 kDa nuclear and cell adhesion-related phosphoprotein; desmosome-associated protein; domain-rich serine protein; DRS; DRSP; melanoma metastasis clone A protein; memA; neutrophil protein; nuclear protein SDK3; pinin; SDK3; SR-like protein
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PNN/DRSP using anti-PNN/DRSP antibody (A01590-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNN/DRSP antigen affinity purified polyclonal antibody (Catalog # A01590-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNN/DRSP at approximately 82KD. The expected band size for PNN/DRSPis at 82KD.
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