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Western Blot All lanes: PNPLA1 antibody at 12ug/ml Lane 1: Mouse spleen tissue Lane 2: Mouse skeletal muscle tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 48, 49 kDa Observed band size: 58 kDa
Western Blot All lanes: PNPLA1 antibody at 12ug/ml Lane 1: Mouse spleen tissue Lane 2: Mouse skeletal muscle tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 48, 49 kDa Observed band size: 58 kDa
Western Blot All lanes: PNPLA1 antibody at 12ug/ml Lane 1: Mouse spleen tissue Lane 2: Mouse skeletal muscle tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 48, 49 kDa Observed band size: 58 kDa

PNPLA1 Antibody

CSB-PA843295LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPNPLA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PNPLA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID285848
  • Target name
    PNPLA1
  • Target description
    patatin like domain 1, omega-hydroxyceramide transacylase
  • Target synonyms
    ARCI10, dJ50J22.1, omega-hydroxyceramide transacylase, patatin like phospholipase domain containing 1, patatin-like phospholipase domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N8W4
  • Protein Name
    Omega-hydroxyceramide transacylase
  • Scientific Description
    Lipid hydrolase. Important in the formation of the epidermal lipid barrier. Plays a role in glycerophospholipid metabolism.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PNPLA1 using anti-PNPLA1 antibody (A09557-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPLA1 antigen affinity purified polyclonal antibody (Catalog # A09557-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNPLA1 at approximately 58 kDa. The expected band size for PNPLA1 is at 58 kDa.
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