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Lane 1: Rat Ear tissue lysates; Lane 2: Rat Smal intestine tissue lysates; Lane 3: Rat Stomach tissue lysates; Lane 4: Rat Kidney tissue lysates probed with PNPLA1 Polyclonal Antibody, Unconjugated (bs-24622R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Ear tissue lysates; Lane 2: Rat Smal intestine tissue lysates; Lane 3: Rat Stomach tissue lysates; Lane 4: Rat Kidney tissue lysates probed with PNPLA1 Polyclonal Antibody, Unconjugated (bs-24622R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Ear tissue lysates; Lane 2: Rat Smal intestine tissue lysates; Lane 3: Rat Stomach tissue lysates; Lane 4: Rat Kidney tissue lysates probed with PNPLA1 Polyclonal Antibody, Unconjugated (bs-24622R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

PNPLA1 Polyclonal Antibody

BS-24622R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetPNPLA1
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Overview

  • Supplier
    Bioss
  • Product Name
    PNPLA1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID285848
  • Target name
    PNPLA1
  • Target description
    patatin like domain 1, omega-hydroxyceramide transacylase
  • Target synonyms
    ARCI10, dJ50J22.1, omega-hydroxyceramide transacylase, patatin like phospholipase domain containing 1, patatin-like phospholipase domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N8W4
  • Protein Name
    Omega-hydroxyceramide transacylase
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of PNPLA1 using anti-PNPLA1 antibody (A09557-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPLA1 antigen affinity purified polyclonal antibody (Catalog # A09557-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNPLA1 at approximately 58 kDa. The expected band size for PNPLA1 is at 58 kDa.
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