Non-transfected (–) and transfected (+) PP2A alpha shRNA-transfected 293T (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![PP2A alpha antibody [HL1447] detects PP2A alpha protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX636919/GTX636919_44704_20221230_ICC_IF_23010219_413.webp "PP2A alpha antibody [HL1447] detects PP2A alpha protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636919/GTX636919_44704_20230217_IHC-P_Dog_23030219_611.webp "PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636919/GTX636919_44704_20230217_IHC-P_Cat_23030219_615.webp "PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636919/GTX636919_44704_20220610_WB_w_23061202_629.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636919/GTX636919_T-44648_20220429_WB_M_R_w_23061202_231.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636919/GTX636919_T-44648_20220527_IHC-P_R_w_23061202_847.webp "PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636919/GTX636919_T-44648_20220527_IHC-P_M_w_23061202_698.webp "PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636919/GTX636919_44704_20230901_WB_Drosophila_23090619_261.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Non-transfected (–) and transfected (+) PP2A alpha shRNA-transfected 293T (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PP2A alpha antibody [HL1447]
GTX636919
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Drosophila, Feline, Human, Mouse, Rat
TargetPPP2CA
Overview
- SupplierGeneTex
- Product NamePP2A alpha antibody [HL1447]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1447
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5515
- Target namePPP2CA
- Target descriptionprotein phosphatase 2 catalytic subunit alpha
- Target synonymsHJS3, NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, protein phosphatase 2, catalytic subunit, alpha isozyme, replication protein C
- HostRabbit
- IsotypeIgG
- Protein IDP67775
- Protein NameSerine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
- Scientific DescriptionThis gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
- ReactivityCanine, Drosophila, Feline, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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