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Mouse heart lysates probed with PPAR alpha Polyclonal Antibody, Unconjugated (bs-23398R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse heart lysates probed with PPAR alpha Polyclonal Antibody, Unconjugated (bs-23398R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse heart lysates probed with PPAR alpha Polyclonal Antibody, Unconjugated (bs-23398R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

PPAR alpha Polyclonal Antibody

BS-23398R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPPARA
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PPAR alpha Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5465
  • Target name
    PPARA
  • Target description
    peroxisome proliferator activated receptor alpha
  • Target synonyms
    hPPAR; NR1C1; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3; peroxisome proliferator-activated receptor alpha; PPAR; PPARalpha; PPAR-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07869
  • Protein Name
    Peroxisome proliferator-activated receptor alpha
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats. Pan Y et al., 2020 Jul 15, Int J Biol Macromol
    Read more
  • Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Dong S et al., 2019 Jun 15, Life Sci
    Read more
  • Fine particles cause the abnormality of cardiac ATP levels via PPARalpha-mediated utilization of fatty acid and glucose using in vivo and in vitro models. Jin X et al., 2019 Jun, Environ Pollut
    Read more
  • Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: Reduction of PPARalpha. Bian X et al., 2019 May, Biochimie
    Read more

Datasheet

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Figure 1. Western blot analysis of PPAR alpha/PPARA using anti-PPAR alpha/PPARA antibody (A00600-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAR alpha/PPARA antigen affinity purified polyclonal antibody (Catalog # A00600-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAR alpha/PPARA at approximately 52 kDa. The expected band size for PPAR alpha/PPARA is at 52 kDa.
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