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PPP2CA Polyclonal Antibody

RD76052A
Reddot Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPPP2CA
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    PPP2CA Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5515
  • Target name
    PPP2CA
  • Target description
    protein phosphatase 2 catalytic subunit alpha
  • Target synonyms
    HJS3, NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, protein phosphatase 2, catalytic subunit, alpha isozyme, replication protein C
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP2CA using anti-PPP2CA antibody (PB9347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2CA antigen affinity purified polyclonal antibody (Catalog # PB9347) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2CA at approximately 34, 36 kDa. The expected band size for PPP2CA is at 36 kDa.
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