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Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti PRAME Polyclonal Antibody, Unconjugated (bs-7004R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti PRAME Polyclonal Antibody, Unconjugated (bs-7004R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti PRAME Polyclonal Antibody, Unconjugated (bs-7004R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

PRAME Polyclonal Antibody

BS-7004R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRAME
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Overview

  • Supplier
    Bioss
  • Product Name
    PRAME Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23532
  • Target name
    PRAME
  • Target description
    PRAME nuclear receptor transcriptional regulator
  • Target synonyms
    CT130, MAPE, OIP-4, OIP4, melanoma antigen preferentially expressed in tumors, Opa-interacting protein OIP4, cancer/testis antigen 130, opa-interacting protein 4, preferentially expressed antigen in melanoma, preferentially expressed antigen of melanoma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78395
  • Protein Name
    Melanoma antigen preferentially expressed in tumors
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PRAME using anti-PRAME antibody (A06628-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAME antigen affinity purified polyclonal antibody (Catalog # A06628-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAME at approximately 58KD. The expected band size for PRAME is at 58KD.
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