WB analysis of whole cell extracts (30 microg lysate) of PANC-1 (Lane 1), HCT 116 (Lane 2), C2C12 (Lane 3), HeLa (Lane 4), SH-SY5Y (Lane 5), K-562 (Lane 6) and Raji (Lane 7) using GTX15796 PRC1 antibody [16F2]. Dilution : 1-3 microg/ml
![ICC/IF analysis of HeLa cells using GTX15796 PRC1 antibody [16F2]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15796/GTX15796_320_ICC-IF_w_23060620_871.webp "ICC/IF analysis of HeLa cells using GTX15796 PRC1 antibody [16F2]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of A2058 Cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15796/GTX15796_321_ICC-IF_w_23060620_442.webp "ICC/IF analysis of A2058 Cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of HeLa cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15796/GTX15796_322_ICC-IF_w_23060620_316.webp "ICC/IF analysis of HeLa cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of U251 cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15796/GTX15796_323_ICC-IF_w_23060620_548.webp "ICC/IF analysis of U251 cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
WB analysis of whole cell extracts (30 microg lysate) of PANC-1 (Lane 1), HCT 116 (Lane 2), C2C12 (Lane 3), HeLa (Lane 4), SH-SY5Y (Lane 5), K-562 (Lane 6) and Raji (Lane 7) using GTX15796 PRC1 antibody [16F2]. Dilution : 1-3 microg/ml
PRC1 antibody [16F2]
GTX15796
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRC1
Overview
- SupplierGeneTex
- Product NamePRC1 antibody [16F2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1-3 microg/ml. ICC/IF: 1-2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID16F2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9055
- Target namePRC1
- Target descriptionprotein regulator of cytokinesis 1
- Target synonymsanaphase spindle elongation 1 homolog; ASE1; protein regulating cytokinesis 1; protein regulator of cytokinesis 1
- HostMouse
- IsotypeIgG2b
- Protein IDO43663
- Protein NameProtein regulator of cytokinesis 1
- Scientific DescriptionThis gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203