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PRC1 Antibody (Preservative Free)

LS-C815688
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRC1 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:1500 - 1:50000), IF (1:1500 - 1:50000), IHC-P (1:2500 - 1:10000), WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9055
  • Target name
    PRC1
  • Target description
    protein regulator of cytokinesis 1
  • Target synonyms
    anaphase spindle elongation 1 homolog; ASE1; protein regulating cytokinesis 1; protein regulator of cytokinesis 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRC1 using anti-PRC1 antibody (PB9814). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: SGC Whole Cell Lysate, Lane 3: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRC1 antigen affinity purified polyclonal antibody (Catalog # PB9814) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRC1 at approximately 75 kDa, 80 kDa. The expected band size for PRC1 is at 75 kDa, 80 kDa.
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