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Detection of endogenous SAPK2 delta Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK2d-5H7 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620
Product group Antibodies
OriGene
SAPK4 (MAPK13) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5H7]AM00138PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMAPK13
  • SizePrice
Detection of Endogenous Scramblase 1. Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Scram1-1E9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK-293 Lane 4: SY5Y Lane 5: Jurkat Lane 6: U937 Lane 7: K562 Lane 8: THP
Product group Antibodies
OriGene
Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E9]AM00139PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetPLSCR1
  • SizePrice
Detection of endogenous shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc-11F6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3
Product group Antibodies
OriGene
SHC (SHC1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11F6]AM00140PU-N
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSHC1
  • SizePrice
Phosphospecificity Whole cell extracts of control (co), EGF-stimulated (EGF) or pervanadate-treated (VH) A549 tumor cells were applied to SDS-PAGE (ca. 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-1E3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
SHC (SHC1) pTyr239/240 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E3]AM00141PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetSHC1
  • SizePrice
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
SHC (SHC1) pTyr317 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15E11]AM00142PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetSHC1
  • SizePrice
Detection of endogenous p66shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc/p66-24E4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
Product group Antibodies
OriGene
SHC (SHC1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24E4]AM00143PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetSHC1
  • SizePrice
Immunoblot Analysis: HeLa cells were cultured under serum-free conditions for 24h and subsequently stimulated with 10 ng/ml EGF. Cells were lysed with RIPA buffer and shc immunoprecipitated with polyclonal anti-shc (Transduction Labs). Immunoprecipitates were separated by SDS-PAGE. Immunoblots were developed using mab shc/p66-6E10 at 1 ug/ml.
Product group Antibodies
OriGene
SHC (SHC1) pSer36 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6E10]AM00144PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetSHC1
  • SizePrice
Product group Antibodies
OriGene
SRC pTyr416 Mouse Monoclonal Antibody [Clone ID: 9A6]AM00145BT-N
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
TargetSRC
  • SizePrice
Phosphospecificity: Whole cell extracts of control (co) and pervanadate stimulated HEK-293 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab src 9A6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: co; Lane 2: pervanadate
Product group Antibodies
OriGene
SRC pTyr416 Mouse Monoclonal Antibody [Clone ID: 9A6]AM00145PU-N
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
TargetSRC
  • SizePrice
Detection of endogenous Src: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00146PU-N Src antibody Clone 11F1 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Product group Antibodies
OriGene
SRC (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11F1]AM00146PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetSRC
  • SizePrice
Phosphospecificity Whole cell extracts of control (1), EGF stimulated (2) or pervanadate treated (3) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab STAT1-12C5 (0.5
Product group Antibodies
OriGene
STAT1 pSer727 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12C5]AM00147PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetSTAT1
  • SizePrice
Product group Antibodies
OriGene
STAT3 pTyr705 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9E12]AM00148BT-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetSTAT3
  • SizePrice
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