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The Binding Activity of CLEC4C with anti-CLEC4C antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLEC4C (CSB-MP855470HUh7) at 2 microg/mL can bind Anti-CLEC4C recombinant antibody, the EC50 is 7.658-12.99 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCLEC4C
IHC image of CSB-RA858415MA1HU diluted at 1 : 100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSOST
IHC image of CSB-RA859530MA1HU diluted at 1 : 100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetGDF15
IHC image of CSB-RA860321MA1HU diluted at 1 : 100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCSF3R
The Binding Activity of Macaca fascicularis CD93 with Anti-CD93 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD93 (CSB-MP4279MOV) at 2 microg/mL can bind Anti-CD93 recombinant antibody. The EC50 is 0.1669-0.3513 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman, Monkey
TargetCD93
IHC image of CSB-RA867113MA1HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL22
Western Blot Positive WB detected in: K562 whole cell lysate All lanes: TUBB1 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTUBB1
The Binding Activity of Human KAAG1 with Anti-KAAG1 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human KAAG1(CSB-EP871385HU) at 2 microg/mL can bind Anti-KAAG1 recombinant antibody.The EC50 is 2.040-2.284 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetKAAG1
The Binding Activity of Macaca fascicularis IL1RAP with Anti-IL1RAP recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL1RAP (CSB-MP5268MOV) at 2 microg/mL can bind Anti-IL1RAP recombinant antibody. The EC50 is 0.9099-1.181 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman, Monkey
TargetIL1RAP
Overlay Peak curve showing A549 cells stained with CSB-RA878853MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetRTN4
IHC image of CSB-RA878859MA1HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetXPNPEP1
IHC image of CSB-RA878942MA1HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCD274