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The Binding Activity of Human MUC17 with Anti-MUC17 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human MUC17 (CSB-MP727848HU) at 2 microg/mL can bind Anti-MUC17 recombinant antibody. The EC50 is 0.9057-1.259 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetMUC17
The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP5140MOV) at 2 microg/mL can bind Anti-CD276 recombinant antibody. The EC50 is 4.299-5.373 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman, Monkey
TargetCD276
The Binding Activity of Human BTLA with Anti-BTLA Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human BTLA(CSB-MP773799HU1)at 2 microg/mL can bind Anti-BTLA recombinant antibody , the EC50 is 11.56-13.00 ng/mL.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetBTLA
Overlay Peak curve showing Hela cells stained with CSB-RA778654A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetPLAT
IHC image of CSB-RA782732A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.69% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSOD2
IHC image of CSB-RA783071A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.17% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCR1
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA783626A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetHRH3
Immunofluorescence staining of A549 with CSB-RA788545A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 519-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetKRT6A
IHC image of CSB-RA792403A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.06% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH3C1
IHC image of CSB-RA796958A0HU diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.67% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDEK
Western Blot Positive WB detected in: Mouse Brain tissue lysate All lanes: CHAT antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCHAT
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA846828A0ENV (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargetmalE