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IHC image of CSB-RA029079A0HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.35% DAB.
Product group Antibodies
Cusabio
DPYSL2 Recombinant Monoclonal AntibodyCSB-RA029079A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDPYSL2
Overlay Peak curve showing HepG2 cells stained with CSB-RA029525A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
FDPS Recombinant Monoclonal AntibodyCSB-RA029525A0HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetFDPS
IHC image of CSB-RA619084MA1HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
FSCN1 Recombinant Monoclonal AntibodyCSB-RA619084MA1HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetFSCN1
IHC image of CSB-RA620753A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.79% DAB.
Product group Antibodies
Cusabio
WDR5 Recombinant Monoclonal AntibodyCSB-RA620753A0HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetWDR5
Immunofluorescence staining of MCF cell with CSB-RA620892MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
Product group Antibodies
Cusabio
SLC1A5 Recombinant Monoclonal AntibodyCSB-RA620892MA1HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetSLC1A5
The Binding Activity of DSG2 with anti-DSG2 antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human DSG2 (CSB-MP622752HU) at 2 microg/mL can bind Anti-DSG2 recombinant antibody, the EC50 is 20.26-38.00 ng/mL.
Product group Antibodies
Cusabio
DSG2 Recombinant Monoclonal AntibodyCSB-RA622752MA1HU
ApplicationsELISA
ReactivityHuman
TargetDSG2
Overlay Peak curve showing Hela cells stained with CSB-RA622928MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 003 events was performed.
Product group Antibodies
Cusabio
GPNMB Recombinant Monoclonal AntibodyCSB-RA622928MA1HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetGPNMB
IHC image of CSB-RA623829MA1HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ACVR2B Recombinant Monoclonal AntibodyCSB-RA623829MA1HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetACVR2B
Immunofluorescence staining of Hela cell with CSB-RA624113MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
HIF1A Recombinant Monoclonal AntibodyCSB-RA624113MA1HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetHIF1A
IHC image of CSB-RA624122MA1HU diluted at 1 : 150 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
AOC3 Recombinant Monoclonal AntibodyCSB-RA624122MA1HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetAOC3
IHC image of CSB-RA644510A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.74% DAB.
Product group Antibodies
Cusabio
HSP90AB1 Recombinant Monoclonal AntibodyCSB-RA644510A0HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetHSP90AB1
Overlay Peak curve showing PC3 cells surface stained with CSB-RA723415MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
NT5E Recombinant Monoclonal AntibodyCSB-RA723415MA1HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetNT5E
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