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ProductsBack/Prion Protein (PrP) antibody [EM-20]
WB analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using GTX80160 Prion Protein (PrP) antibody [EM-20]. CJD positive patient has proteinase K resistent prion protein. Lane 1, 4 : With proteinase K treatment Lane 2, 3 : Without proteinase K treatment
WB analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using GTX80160 Prion Protein (PrP) antibody [EM-20]. CJD positive patient has proteinase K resistent prion protein. Lane 1, 4 : With proteinase K treatment Lane 2, 3 : Without proteinase K treatment
WB analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using GTX80160 Prion Protein (PrP) antibody [EM-20]. CJD positive patient has proteinase K resistent prion protein. Lane 1, 4 : With proteinase K treatment Lane 2, 3 : Without proteinase K treatment

Prion Protein (PrP) antibody [EM-20]

GTX80160
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPRNP
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Overview

  • Supplier
    GeneTex
  • Product Name
    Prion Protein (PrP) antibody [EM-20] - Orthogonal Validated
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    EM-20
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5621
  • Target name
    PRNP
  • Target description
    prion protein
  • Target synonyms
    alternative prion protein; AltPrP; ASCR; CD230; CD230 antigen; CJD; GSS; KURU; major prion protein; p27-30; prion-related protein; PRIP; PrP; PrP27-30; PrP33-35C; PrPc
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDF7VJQ1
  • Protein Name
    Alternative prion protein
  • Scientific Description
    The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRNP using anti-PRNP antibody (PB9783). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRNP antigen affinity purified polyclonal antibody (Catalog # PB9783) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRNP at approximately 30 kDa. The expected band size for PRNP is at 30 kDa.
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