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ProductsBack/PRKCH / PKC-Eta Antibody (aa30-389)

PRKCH / PKC-Eta Antibody (aa30-389)

LS-C407769
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPRKCH
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRKCH / PKC-Eta Antibody (aa30-389)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5583
  • Target name
    PRKCH
  • Target description
    protein kinase C eta
  • Target synonyms
    PKC-L, PKCL, PRKCL, nPKC-eta, uORF2, protein kinase C eta type, Protein uPEP2
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PKC eta using anti-PKC eta antibody (PB9556). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate Lane 4: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC eta antigen affinity purified polyclonal antibody (Catalog # PB9556) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC eta at approximately 78KD. The expected band size for PKC eta is at 78KD.
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