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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT3610] (GTX628488) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT3610] (GTX628488) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT3610] (GTX628488) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

PRMT5 antibody [GT3610]

GTX628488
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Rat
TargetPRMT5
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRMT5 antibody [GT3610]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT3610
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10419
  • Target name
    PRMT5
  • Target description
    protein arginine methyltransferase 5
  • Target synonyms
    HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs, protein arginine N-methyltransferase 5, 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, SKB1 homolog, histone-arginine N-methyltransferase PRMT5, jak-binding protein 1, shk1 kinase-binding protein 1 homolog
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDO14744
  • Protein Name
    Protein arginine N-methyltransferase 5
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yang M, Lin X, Segers F, et al. OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation. Cell Rep. 2020,30(12):4165-4178.e7. doi: 10.1016/j.celrep.2020.02.063
    Read this paper

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Figure 1. Western blot analysis of PRMT5 using anti-PRMT5 antibody (A00635-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT5 antigen affinity purified polyclonal antibody (Catalog # A00635-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT5 at approximately 72KD. The expected band size for PRMT5 is at 72KD.
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