More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PRMT5 antibody [N1C1]
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5(GTX116004) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5(GTX116004) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5(GTX116004) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

PRMT5 antibody [N1C1]

GTX116004
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPRMT5
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    PRMT5 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.31 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10419
  • Target name
    PRMT5
  • Target description
    protein arginine methyltransferase 5
  • Target synonyms
    HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs, protein arginine N-methyltransferase 5, 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, SKB1 homolog, histone-arginine N-methyltransferase PRMT5, jak-binding protein 1, shk1 kinase-binding protein 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14744
  • Protein Name
    Protein arginine N-methyltransferase 5
  • Scientific Description
    Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 Arg-3 during germ cell development. Methylates histone H3 Arg-8, which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sun X, Zhou J, Zhang Z. Cullin 4A/protein arginine methyltransferase 5 (CUL4A/PRMT5) promotes cell malignant phenotypes and tumor growth in nasopharyngeal carcinoma. Bioengineered. 2022,13(4):8712-8723. doi: 10.1080/21655979.2022.2054756
    Read this paper
  • Cai S, Wang P, Xie T, et al. Histone H4R3 symmetric di-methylation by Prmt5 protects against cardiac hypertrophy via regulation of Filip1L/β-catenin. Pharmacol Res. 2020,161:105104. doi: 10.1016/j.phrs.2020.105104
    Read this paper
  • Liu J, Liu Y, Shao J, et al. Zeb1 is important for proper cleavage plane orientation of dividing progenitors and neuronal migration in the mouse neocortex. Cell Death Differ. 2019,26(11):2479-2492. doi: 10.1038/s41418-019-0314-9
    Read this paper
  • Liu X, Zhang J, Liu L, et al. Protein arginine methyltransferase 5-mediated epigenetic silencing of IRX1 contributes to tumorigenicity and metastasis of gastric cancer. Biochim Biophys Acta Mol Basis Dis. 2018,1864(9 Pt B):2835-2844. doi: 10.1016/j.bbadis.2018.05.015
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-PRMT5 [RAB-C136]Ab01705-1.1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetPRMT5
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PRMT5 Antibody144-01520
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetPRMT5
  • SizePrice
Figure 1. Western blot analysis of PRMT5 using anti-PRMT5 antibody (A00635-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT5 antigen affinity purified polyclonal antibody (Catalog # A00635-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT5 at approximately 72KD. The expected band size for PRMT5 is at 72KD.
Product group Antibodies
Boster Bio
Anti-PRMT5 Antibody Picoband(r)A00635-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPRMT5
  • SizePrice
PRMT5 antibody [N3C3] detects PRMT5 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT5 stained by PRMT5 antibody [N3C3] (GTX115409) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Product group Antibodies
GeneTex
PRMT5 antibody [N3C3]GTX115409
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetPRMT5
  • SizePrice
PRMT5 antibody [N1N3] detects PRMT5 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PRMT5 antibody [N1N3] (GTX115994) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
GeneTex
PRMT5 antibody [N1N3]GTX115994
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetPRMT5
  • SizePrice
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT3610] (GTX628488) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
References
GeneTex
PRMT5 antibody [GT3610]GTX628488
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Rat
TargetPRMT5
  • SizePrice
Product group Antibodies
Biomatik
Prmt5 Recombinant AntibodyCAC12525
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPRMT5
  • SizePrice
Product group Antibodies
Bioss
PRMT5 Recombinant AntibodyBSM-60525R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetPRMT5
  • SizePrice
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA018734LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
PRMT5 AntibodyCSB-PA018734LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPRMT5
  • SizePrice
Anti-PRMT5 Antibody
Product group Antibodies
Antibodies.com
Anti-PRMT5 AntibodyA30085
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice