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WB analysis of various sample lysates using GTX33428 PRODH antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33428 PRODH antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33428 PRODH antibody. Dilution : 1:1000 Loading : 25microg per lane

PRODH antibody

GTX33428
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPRODH
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRODH antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5625
  • Target name
    PRODH
  • Target description
    proline dehydrogenase 1
  • Target synonyms
    HSPOX2; p53-induced gene 6 protein; PIG6; POX; PRODH1; PRODH2; proline dehydrogenase (oxidase) 1; proline dehydrogenase 1, mitochondrial; proline oxidase 2; proline oxidase, mitochondrial; TP53I6; tumor protein p53 inducible protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43272
  • Protein Name
    Proline dehydrogenase 1, mitochondrial
  • Scientific Description
    This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Proline improves cardiac remodeling following myocardial infarction and attenuates cardiomyocyte apoptosis via redox regulation. Wang J et al., 2020 Aug, Biochem Pharmacol
    Read more

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Figure 1. Western blot analysis of PRODH using anti-PRODH antibody (A03231-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRODH antigen affinity purified polyclonal antibody (Catalog # A03231-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRODH at approximately 69 kDa. The expected band size for PRODH is at 69 kDa.
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