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Goat anti-PRODH

EB07166
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPRODH
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PRODH Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5625
  • Target name
    PRODH
  • Target description
    proline dehydrogenase 1
  • Target synonyms
    HSPOX2, PIG6, POX, PRODH1, TP53I6, proline dehydrogenase 1, mitochondrial, p53 Inducible gene 6, p53-induced gene 6 protein, proline dehydrogenase (oxidase) 1, proline oxidase 2, proline oxidase, mitochondrial, tumor protein p53 inducible protein 6
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_057419.2. GeneIDs all Nonhuman: 19125 (mouse);. Purification: Antigen affinity purified. Names and symbols: PRODH; proline dehydrogenase (oxidase) 1 ; HSPOX2; PIG6; PRODH1; PRODH2; SCZD4; TP53I6; FLJ33744; MGC148078; MGC148079; Proline oxidase, mitochondrial; p53 induced protein; proline dehydrogenase (proline oxidase ); proline oxidase 2; tumor protein p53 ind
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Tołoczko-Iwaniuk N, Dziemiańczyk-Pakieła D, Celińska-Janowicz K, et al. Proline-Dependent Induction of Apoptosis in Oral Squamous Cell Carcinoma (OSCC)-The Effect of Celecoxib. Cancers (Basel). 2020,12(1). doi: 10.3390/cancers12010136
    Read this paper
  • Zareba I, Celinska-Janowicz K, Surazynski A, et al. Proline oxidase silencing induces proline-dependent pro-survival pathways in MCF-7 cells. Oncotarget. 2018,9(17):13748-13757. doi: 10.18632/oncotarget.24466
    Read this paper

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Figure 1. Western blot analysis of PRODH using anti-PRODH antibody (A03231-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRODH antigen affinity purified polyclonal antibody (Catalog # A03231-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRODH at approximately 69 kDa. The expected band size for PRODH is at 69 kDa.
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