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ProductsBack/PRODH Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PRODH Polyclonal Antibody, Unconjugated (bs-5813R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PRODH Polyclonal Antibody, Unconjugated (bs-5813R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PRODH Polyclonal Antibody, Unconjugated (bs-5813R) at 1:400 overnight at 4°C, DAB staining.

PRODH Polyclonal Antibody

BS-5813R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRODH
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PRODH Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5625
  • Target name
    PRODH
  • Target description
    proline dehydrogenase 1
  • Target synonyms
    HSPOX2; p53-induced gene 6 protein; PIG6; POX; PRODH1; PRODH2; proline dehydrogenase (oxidase) 1; proline dehydrogenase 1, mitochondrial; proline oxidase 2; proline oxidase, mitochondrial; TP53I6; tumor protein p53 inducible protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43272
  • Protein Name
    Proline dehydrogenase 1, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PRODH using anti-PRODH antibody (A03231-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRODH antigen affinity purified polyclonal antibody (Catalog # A03231-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRODH at approximately 69 kDa. The expected band size for PRODH is at 69 kDa.
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