IHC-P analysis of human tonsil tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IHC-P analysis of human uterus tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX30164/GTX30164_1330_IHC-P_w_23060722_696.webp "IHC-P analysis of human uterus tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human breast carcinoma tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX30164/GTX30164_1331_IHC-P_w_23060722_571.webp "IHC-P analysis of human breast carcinoma tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20")
![WB analysis of 20 ug of nuclear (N) or cytoplasmic (C) of T47D cell lysate untreated (-) or stimulated (+) with 100 nM promegestone (R5020) for 1 hour using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154] at 4 microg/ml (upper panel) or a Progesterone Receptor mouse monoclonal antibody (lower pane) . This antibody detects both phosphorylated isoform A and isoform B.](https://www.genetex.com/upload/website/prouct_img/normal/GTX30164/GTX30164_1842_WB_w_23060722_597.webp "WB analysis of 20 ug of nuclear (N) or cytoplasmic (C) of T47D cell lysate untreated (-) or stimulated (+) with 100 nM promegestone (R5020) for 1 hour using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154] at 4 microg/ml (upper panel) or a Progesterone Receptor mouse monoclonal antibody (lower pane) . This antibody detects both phosphorylated isoform A and isoform B.")
![ICC/IF analysis of T47D cells using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight](https://www.genetex.com/upload/website/prouct_img/normal/GTX30164/GTX30164_722_ICC-IF_w_23060722_756.webp "ICC/IF analysis of T47D cells using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight")
![ICC/IF analysis of untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel) using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute](https://www.genetex.com/upload/website/prouct_img/normal/GTX30164/GTX30164_723_ICC-IF_w_23060722_451.webp "ICC/IF analysis of untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel) using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute")
IHC-P analysis of human tonsil tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
Progesterone Receptor (phospho Ser190) antibody [1154]
GTX30164
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPGR
Overview
- SupplierGeneTex
- Product NameProgesterone Receptor (phospho Ser190) antibody [1154] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 4 microg/ml. ICC/IF: 1:10-1:100. IHC-P: 2-4 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1154
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5241
- Target namePGR
- Target descriptionprogesterone receptor
- Target synonymsNR3C3; nuclear receptor subfamily 3 group C member 3; PR; progesterone receptor
- HostMouse
- IsotypeIgG1
- Protein IDP06401
- Protein NameProgesterone receptor
- Scientific DescriptionThis gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203