ELISA analysis of antigen using GTX60495 Prohibitin antibody [5H7]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
![FACS analysis of MCF-7 cells using GTX60495 Prohibitin antibody [5H7]. Blue : Prohibitin Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60495/GTX60495_20170912_FACS_w_23061123_320.webp "FACS analysis of MCF-7 cells using GTX60495 Prohibitin antibody [5H7]. Blue : Prohibitin Red : negative control")
![ICC/IF analysis of NIH3T3 cells using GTX60495 Prohibitin antibody [5H7]. Green : Prohibitin Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60495/GTX60495_20170912_ICCIF_w_23061123_328.webp "ICC/IF analysis of NIH3T3 cells using GTX60495 Prohibitin antibody [5H7]. Green : Prohibitin Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments")
![IHC-P analysis of lung cancer tissue using GTX60495 Prohibitin antibody [5H7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60495/GTX60495_20170912_IHC-P_1_w_23061123_827.webp "IHC-P analysis of lung cancer tissue using GTX60495 Prohibitin antibody [5H7].")
![IHC-P analysis of rectum cancer tissue using GTX60495 Prohibitin antibody [5H7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60495/GTX60495_20170912_IHC-P_2_w_23061123_881.webp "IHC-P analysis of rectum cancer tissue using GTX60495 Prohibitin antibody [5H7].")
![IHC-P analysis of stomach cancer tissue using GTX60495 Prohibitin antibody [5H7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60495/GTX60495_20170912_IHC-P_3_w_23061123_698.webp "IHC-P analysis of stomach cancer tissue using GTX60495 Prohibitin antibody [5H7].")
![IHC-P analysis of liver cancer tissue using GTX60495 Prohibitin antibody [5H7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60495/GTX60495_20170912_IHC-P_w_23061123_840.webp "IHC-P analysis of liver cancer tissue using GTX60495 Prohibitin antibody [5H7].")
![WB analysis of human Prohibitin (AA: 68-259) recombinant protein using GTX60495 Prohibitin antibody [5H7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60495/GTX60495_20170912_WB_1_w_23061123_995.webp "WB analysis of human Prohibitin (AA: 68-259) recombinant protein using GTX60495 Prohibitin antibody [5H7].")
![WB analysis of A431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A549 (6), NIH3T3 (7), Cos7 (8) and PC-12 (9) cell lysate using GTX60495 Prohibitin antibody [5H7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60495/GTX60495_20170912_WB_w_23061123_946.webp "WB analysis of A431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A549 (6), NIH3T3 (7), Cos7 (8) and PC-12 (9) cell lysate using GTX60495 Prohibitin antibody [5H7].")
ELISA analysis of antigen using GTX60495 Prohibitin antibody [5H7]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
Prohibitin antibody [5H7]
GTX60495
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetPHB1
Overview
- SupplierGeneTex
- Product NameProhibitin antibody [5H7]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5H7
- ConjugateUnconjugated
- Gene ID5245
- Target namePHB1
- Target descriptionprohibitin 1
- Target synonymsBAP32, HEL-215, HEL-S-54e, PHB, prohibitin 1, epididymis luminal protein 215, epididymis secretory sperm binding protein Li 54e, prohibitin
- HostMouse
- IsotypeIgG1
- Protein IDP35232
- Protein NameProhibitin 1
- Scientific DescriptionThis gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3 UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Buehler U, Schulenburg K, Yurugi H, et al. Targeting prohibitins at the cell surface prevents Th17-mediated autoimmunity. EMBO J. 2018,37(16). doi: 10.15252/embj.201899429Read this paper
Datasheet
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