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ProductsBack/Properdin (CFP) Mouse Monoclonal Antibody [Clone ID: 10-24]

Properdin (CFP) Mouse Monoclonal Antibody [Clone ID: 10-24]

AM01338PU-N
OriGene
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCFP
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Properdin (CFP) Mouse Monoclonal Antibody [Clone ID: 10-24]
  • Delivery Days Customer
    14
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10/24/2024 12:00:00 AM
  • Gene ID5199
  • Target name
    CFP
  • Target description
    complement factor properdin
  • Target synonyms
    BFD, PFC, PFD, PROPERDIN, properdin, complement factor P, properdin P factor, complement
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP27918
  • Protein Name
    Properdin
  • Scientific Description
    Properdin (CFP) mouse monoclonal antibody, clone 10-24, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CFP using anti-CFP antibody (A00852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-937 whole cell lysate, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFP antigen affinity purified polyclonal antibody (Catalog # A00852-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CFP at approximately 51KD. The expected band size for CFP is at 51KD.
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