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Proteassemblin (B-1): m-IgGkappa BP-HRP Bundle

SC-523877
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOMP
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Proteassemblin (B-1): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID51371
  • Target name
    POMP
  • Target description
    proteasome maturation protein
  • Target synonyms
    C13orf12, HSPC014, PNAS-110, PRAAS2, UMP1, proteasome maturation protein, 2510048O06Rik, hUMP1, proteassemblin, protein UMP1 homolog, voltage-gated K channel beta subunit 4.1, voltage-gated potassium channel beta subunit 4.1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POMP using anti-POMP antibody (A04837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMP antigen affinity purified polyclonal antibody (Catalog # A04837-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMP at approximately 16 kDa. The expected band size for POMP is at 16 kDa.
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