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Western Blot Positive WB detected in: HepG2 whole cell lysate, A2780 whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate All lanes: PRSS8 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 31 kDa Observed band size: 70 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A2780 whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate All lanes: PRSS8 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 31 kDa Observed band size: 70 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A2780 whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate All lanes: PRSS8 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 31 kDa Observed band size: 70 kDa

PRSS8 Antibody

CSB-PA623091LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPRSS8
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRSS8 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5652
  • Target name
    PRSS8
  • Target description
    serine protease 8
  • Target synonyms
    CAP1; channel-activating protease 1; channel-activating serine protease 1; prostasin; protease, serine 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16651
  • Protein Name
    Prostasin
  • Scientific Description
    Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRSS8 using anti-PRSS8 antibody (A05561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS8 antigen affinity purified polyclonal antibody (Catalog # A05561-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS8 at approximately 38 kDa. The expected band size for PRSS8 is at 36 kDa.
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