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PRSS8 Antibody (YA1761, Rabbit)

HY-P82016
MedChem Express
TargetPRSS8
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PRSS8 Antibody (YA1761, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5652
  • Target name
    PRSS8
  • Target description
    serine protease 8
  • Target synonyms
    CAP1, PROSTASIN, prostasin, channel-activating protease 1, channel-activating serine protease 1, protease, serine 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PRSS8 Antibody (YA1761) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRSS8.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRSS8 using anti-PRSS8 antibody (A05561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS8 antigen affinity purified polyclonal antibody (Catalog # A05561-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS8 at approximately 38 kDa. The expected band size for PRSS8 is at 36 kDa.
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TargetPRSS8
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Lane 1: Mouse Muscle lysates; Lane 2: Rat Muscle lysates probed with PRSS8 Polyclonal Antibody, Unconjugated (bs-6064R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Western Blot Positive WB detected in: HepG2 whole cell lysate, A2780 whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate All lanes: PRSS8 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 31 kDa Observed band size: 70 kDa
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Immunohistochemical staining of human rectum shows strong positivity in apical membrane in glandular cells.
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Sample(30 ug whole cell lysate) A:HeLa S3(GTX14654) B:Raji (GTX27908) 10% SDS PAGE GTX103976 diluted at 1:1000
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