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Western Blot analysis of 3T3 cells using Saposin Polyclonal Antibody
Western Blot analysis of 3T3 cells using Saposin Polyclonal Antibody
Western Blot analysis of 3T3 cells using Saposin Polyclonal Antibody

PSAP Antibody

CSB-PA040013
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPSAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSAP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5660
  • Target name
    PSAP
  • Target description
    prosaposin
  • Target synonyms
    GLBA, PARK24, PSAPD, SAP1, SAP2, prosaposin, precursor of saposins, proactivator polypeptide, saposin-A, saposin-B, saposin-C, saposin-D, sphingolipid activator protein-1, sphingolipid activator protein-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07602
  • Protein Name
    Prosaposin
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSAP using anti-PSAP antibody (A00937-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSAP antigen affinity purified polyclonal antibody (Catalog # A00937-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSAP at approximately 70 kDa. The expected band size for PSAP is at 58 kDa.
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