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PSMB6 Antibody

CSB-PA018884GA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMB6
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMB6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5694
  • Target name
    PSMB6
  • Target description
    proteasome 20S subunit beta 6
  • Target synonyms
    DELTA; LMPY; macropain delta chain; multicatalytic endopeptidase complex delta chain; proteasome (prosome, macropain) subunit, beta type, 6; proteasome catalytic subunit 1; proteasome delta chain; proteasome subunit beta 6; proteasome subunit beta type-6; proteasome subunit beta1; proteasome subunit delta; proteasome subunit Y; PSY large multifunctional protease Y; Y
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28072
  • Protein Name
    Proteasome subunit beta type-6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Proteasome 20S Beta 6/PSMB6 using anti-Proteasome 20S Beta 6/PSMB6 antibody (A07705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat lung tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse lung tissue lysates, Lane 14: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Proteasome 20S Beta 6/PSMB6 antigen affinity purified polyclonal antibody (Catalog # A07705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Proteasome 20S Beta 6/PSMB6 at approximately 22 kDa. The expected band size for Proteasome 20S Beta 6/PSMB6 is at 22 kDa.
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