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PSMB6 Antibody

LS-C667981
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPSMB6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PSMB6 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of PSMB6 expression in Hela (A), HepG2 (B), MCF7 (C) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of PSMB6 expression in Hela (A), HepG2 (B), MCF7 (C) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5694
  • Target name
    PSMB6
  • Target description
    proteasome 20S subunit beta 6
  • Target synonyms
    DELTA, LMPY, proteasome subunit beta type-6, PSY large multifunctional protease Y, beta-1, macropain delta chain, multicatalytic endopeptidase complex delta chain, proteasome (prosome, macropain) subunit, beta type, 6, proteasome catalytic subunit 1, proteasome delta chain, proteasome subunit Y, proteasome subunit beta 6, proteasome subunit beta-1, proteasome subunit beta1, proteasome subunit delta
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Proteasome 20S Beta 6/PSMB6 using anti-Proteasome 20S Beta 6/PSMB6 antibody (A07705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat lung tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse lung tissue lysates, Lane 14: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Proteasome 20S Beta 6/PSMB6 antigen affinity purified polyclonal antibody (Catalog # A07705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Proteasome 20S Beta 6/PSMB6 at approximately 22 kDa. The expected band size for Proteasome 20S Beta 6/PSMB6 is at 22 kDa.
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