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PSME2 Antibody Pair

CSB-EAP01134
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Chicken, Goat, Hamster, Human, Mouse, Porcine, Rabbit, Sheep
TargetPSME2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSME2 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 1.6ug/ml and the detection antibody at a concentration of 0.34ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID5721
  • Target name
    PSME2
  • Target description
    proteasome activator subunit 2
  • Target synonyms
    PA28B, PA28beta, REGbeta, proteasome activator complex subunit 2, 11S regulator complex beta subunit, 11S regulator complex subunit beta, MCP activator, 31-kD subunit, REG-beta, activator of multicatalytic protease subunit 2, cell migration-inducing protein 22, proteasome (prosome, macropain) activator subunit 2 (PA28 beta), proteasome activator 28 subunit beta, proteasome activator 28-beta, proteasome activator hPA28 subunit beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UL46
  • Protein Name
    Proteasome activator complex subunit 2
  • Reactivity
    Bovine, Canine, Chicken, Goat, Hamster, Human, Mouse, Porcine, Rabbit, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of PSME2 using anti-PSME2 antibody (A07053-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: rat C6 whole cell lyastes, Lane 8: mouse SP2/0 whole cell lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSME2 antigen affinity purified polyclonal antibody (Catalog # A07053-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSME2 at approximately 32KD. The expected band size for PSME2 is at 27KD.
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