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IHC-P analysis of human brain, glioblastoma tissue using GTX13381 PTGER3 antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human brain, glioblastoma tissue using GTX13381 PTGER3 antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human brain, glioblastoma tissue using GTX13381 PTGER3 antibody. Antigen retrieval : Heat-induced antigen retrieval

PTGER3 antibody

GTX13381
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPTGER3
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Overview

  • Supplier
    GeneTex
  • Product Name
    PTGER3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1 - 3 microg/ml. IHC-P: 1 - 3 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5733
  • Target name
    PTGER3
  • Target description
    prostaglandin E receptor 3
  • Target synonyms
    EP3; EP3e; EP3-I; EP3-II; EP3-III; EP3-IV; EP3-VI; lnc003875; PGE receptor, EP3 subtype; PGE2 receptor EP3 subtype; PGE2-R; prostaglandin E receptor 3 (subtype EP3); prostaglandin E2 receptor EP3 subtype; prostaglandin receptor (PGE-2); prostanoid EP3 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43115
  • Protein Name
    Prostaglandin E2 receptor EP3 subtype
  • Scientific Description
    The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGER3 using anti-PTGER3 antibody (A02548-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER3 antigen affinity purified polyclonal antibody (Catalog # A02548-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER3 at approximately 48 kDa. The expected band size for PTGER3 is at 43 kDa.
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