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Western Blot analysis of HepG2 cells using EP3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using EP3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using EP3 Polyclonal Antibody

PTGER3 Antibody

CSB-PA002352
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPTGER3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTGER3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5733
  • Target name
    PTGER3
  • Target description
    prostaglandin E receptor 3
  • Target synonyms
    EP3; EP3e; EP3-I; EP3-II; EP3-III; EP3-IV; EP3-VI; lnc003875; PGE receptor, EP3 subtype; PGE2 receptor EP3 subtype; PGE2-R; prostaglandin E receptor 3 (subtype EP3); prostaglandin E2 receptor EP3 subtype; prostaglandin receptor (PGE-2); prostanoid EP3 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43115
  • Protein Name
    Prostaglandin E2 receptor EP3 subtype
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTGER3 using anti-PTGER3 antibody (A02548-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER3 antigen affinity purified polyclonal antibody (Catalog # A02548-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER3 at approximately 48 kDa. The expected band size for PTGER3 is at 43 kDa.
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