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PTGER3 / EP3 Antibody

LS-C408750
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPTGER3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PTGER3 / EP3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 40kDa/41kDa/42kDa/43kDa/44kDa/46kDa/47kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:20 - 1:200), WB (1:200 - 1:2000) The predicted MW is 40kDa/41kDa/42kDa/43kDa/44kDa/46kDa/47kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5733
  • Target name
    PTGER3
  • Target description
    prostaglandin E receptor 3
  • Target synonyms
    EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3-VI, EP3e, PGE2-R, lnc003875, prostaglandin E2 receptor EP3 subtype, PGE receptor, EP3 subtype, PGE2 receptor EP3 subtype, prostaglandin E receptor 3 (subtype EP3), prostaglandin receptor (PGE-2), prostanoid EP3 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGER3 using anti-PTGER3 antibody (A02548-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER3 antigen affinity purified polyclonal antibody (Catalog # A02548-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER3 at approximately 48 kDa. The expected band size for PTGER3 is at 43 kDa.
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