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Immunohistochemistry of paraffin-embedded Human liver cancer tissue using COX2 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using COX2 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using COX2 Polyclonal Antibody at dilution 1:40

PTGS2 Polyclonal Antibody

E-AB-17010
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PTGS2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGS2 using anti-PTGS2 antibody (A00084-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7(-LPS) whole cell lysates, Lane 3: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody (Catalog # A00084-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75 kDa. The expected band size for PTGS2 is at 69 kDa.
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Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband(r) AntibodyA00084-2-CARRIER-FREE
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TargetPTGS2
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Product group Antibodies
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Product group Antibodies
Biomatik
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TargetPTGS2
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Product group Antibodies
Cusabio
PTGS2 Monoclonal AntibodyCSB-MA000320
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MCF-7 cells were stained with Cyclooxygenase Polyclonal Antibody, Unconjugated(bs-10411R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Product group Antibodies
References
Bioss
Cyclooxygenase 2 Polyclonal AntibodyBS-10411R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPTGS2
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Product group Antibodies
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TargetPTGS2
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TargetPTGS2
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Immunohistochemical staining of human seminal vesicle shows strong cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
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References
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Goat anti-COX2 / PTGS2 AntibodyEB05286
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Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
References
GeneTex
COX2 antibody [C3], C-termGTX100656
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