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WB analysis of K562 cell lysate using GTX84989 PUMA antibody [10D4G7]. Working concentration : (A) 2.5 and (B) 5 microg/ml
WB analysis of K562 cell lysate using GTX84989 PUMA antibody [10D4G7]. Working concentration : (A) 2.5 and (B) 5 microg/ml
WB analysis of K562 cell lysate using GTX84989 PUMA antibody [10D4G7]. Working concentration : (A) 2.5 and (B) 5 microg/ml

PUMA antibody [10D4G7]

GTX84989
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetBBC3
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Overview

  • Supplier
    GeneTex
  • Product Name
    PUMA antibody [10D4G7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 2.5 to 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10D4G7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27113
  • Target name
    BBC3
  • Target description
    BCL2 binding component 3
  • Target synonyms
    bcl-2-binding component 3; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; p53-upregulated modulator of apoptosis; PUMA
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96PG8
  • Protein Name
    Bcl-2-binding component 3
  • Scientific Description
    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PUMA/BBC3 using anti-PUMA/BBC3 antibody (A04899-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUMA/BBC3 antigen affinity purified polyclonal antibody (Catalog # A04899-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUMA/BBC3 at approximately 18 kDa. The expected band size for PUMA/BBC3 is at 18 kDa.
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