WB analysis of K562 cell lysate using GTX84991 PUMA antibody [2A9G5]. Working concentration : (A) 2.5 and (B) 5 microg/ml
WB analysis of K562 cell lysate using GTX84991 PUMA antibody [2A9G5]. Working concentration : (A) 2.5 and (B) 5 microg/ml
PUMA antibody [2A9G5]
GTX84991
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBBC3
Overview
- SupplierGeneTex
- Product NamePUMA antibody [2A9G5]
- Delivery Days Customer9
- Application Supplier NoteWB: 2.5 to 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A9G5
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID27113
- Target nameBBC3
- Target descriptionBCL2 binding component 3
- Target synonymsbcl-2-binding component 3; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; p53-upregulated modulator of apoptosis; PUMA
- HostMouse
- IsotypeIgG1
- Protein IDQ96PG8
- Protein NameBcl-2-binding component 3
- Scientific DescriptionApoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203